US 9,474,797 B1 · African swine fever virus georgia strain adapted to efficiently grow in the vero cell line

Abstract

We have developed an ASFV Georgia strain adapted to grow in Vero cell line. The resulting virus, ASF-GVAV, efficiently grows in Vero cells although it still is able to significantly replicate in primary cell cultures of swine macrophages. ASF-GVAV virus was successfully used as parental virus to develop several recombinant ASF viruses. The development of an ASFV adapted to grow in an established cell line is a significant advance for research and development of vaccine candidate strains using genetic manipulation based in the process of homologous recombination. The GVAVS can be utilized as a basis for large scale production of ASF vaccines.

Claims7

Dependent claims

An African Swine Fever-Georgia Virus (ASF-GV) encoded by a mutant DNA molecule having the nucleotide sequence of SEQ ID NO:

2 except for a deletion between nucleotide positions 178,643 and 182,578 producing the deletion of the following ORFs:
MGF 505-11L, MGF 100-1L, I7L, I8L, ASFV G ACD 01870, I9R, I10L, and I11L and one or more of the following changes:
(i) nucleotide insertions (T and A) in the non-coding region at position 434 and 441, respectively;
(ii) a double nucleotide (TT) deletion and a nucleotide insertion (T) at positions 1602 and 1620, respectively;
(iii) an amino acid substitution (Cys131Tyr in ORF EP424R) in nucleotide position 71002;
(iv) a synonymous (A to G) substitution at nucleotide position 97391 inside ORF B438L;
(v) an amino acid substitution (Gly257Ser in ORF CP530R) in position 126174;
(vi) an amino acid substitution (Gly127Glu in ORF E199L) in position 166065;
and a nucleotide deletion (T), a nucleotide substitution (T to C) and a nucleotide insertion (T) in a non coding region at nucleotide positions 182582, 182 591 and 183303, respectively.

6 more elements

Dependencies are inferred from the claim text.

African swine fever virus georgia strain adapted to efficiently grow in the vero cell line

Life of this patent

African swine fever virus georgia strain adapted to efficiently grow in the vero cell line

Life of this patent

Abstract

Claims7