Systems and methods for sample use maximization

A method of measuring an analyte concentration in a sample fluid comprising:

• (a) providing the sample contained in a container dimensioned with a plurality of distinct widths to permit transmission of light along a plurality of varying path lengths that correspond to the plurality of distinct widths;
• (b) illuminating the container along at least one of the plurality of path lengths;
• and
• (c) imaging the container to measure a first light intensity transmitted across said at least one of the plurality of path lengths, for the determination of the concentration of the analyte based on the measured first light intensity;
• (d) imaging the same container to measure a second light intensity transmitted across another path length corresponding to another distinct width of the container;
• (e) comparing said first light intensity and the second light intensity;
• and
• (f) determining an analyte concentration based on said comparing step.