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US 9,435,817 B2Granted
Detection of synthetic cannabinoids
AssigneeRandox Laboratories Limited
InventorsElouard Benchikh, Stephen Peter Fitzgerald, Paul John Innocenzi, Philip Andrew Lowry, Ivan Robert McConnell
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Abstract
The invention describes methods and kits for detecting and determining current and future synthetic cannabinoids from the CP family. Unique antibodies derived from novel immunogens enable said methods and kits.
Claims2
An antibody that binds to (±)-CP-47,497 (C7 homologue) and (±)-CP-47,497 (C8 homologue),
- wherein the antibody has a cross-reactivity presented as B/B0 of ≦50% against (±)-CP-47,497 (C7 homologue) at a concentration of 100 ng/ml, and a cross-reactivity presented as B/B0 of ≦50% against (±)-CP-47,497 (C8 homologue) at a concentration of 100 ng/ml, and
- wherein the antibody has a cross-reactivity presented as B/B0 of greater than 80% against each one of the cross-reactants selected from the group consisting of (±)-CP-47,497 (C7 para quinone analogue), (±)-CP-55,940, (−)-CP 55,940, (+)-CP 55, 940, HU-210, HU-211 (dexanabinol), HU-308, delta9-THC, and (−)-11-nor-9-carboxy-delta9-THC, at a cross-reactant concentration of 100 ng/ml,
- as determined using (±)-CP-47,497 (C8 homologue) as a standard and Tracer 1,
- wherein Tracer 1 has the following structural formula:
- wherein HRP is Horse Radish Peroxidase,
- wherein B0 is absorbance at 450 nm at zero ng/ml standard concentration, and
- wherein B is absorbance at 450 nm at predetermined variable standard concentrations.
4 more elements
An antibody raised against an immunogen of the following structure:
- wherein the accm is an antigenicity conferring carrier material;
- wherein the crosslinker is a functionalised linking group joining the accm to the remainder of the molecule, the crosslinker forming a double bond to the cyclohexyl ring;
- wherein m is 3;
- wherein the antibody has a cross-reactivity presented as B/B0 of ≦50% against (±) CP-47,497 (C7 homologue) at a concentration of 100 ng/ml and a cross-reactivity presented as B/B0 of ≦50% against (±)-CP-47,497 (C8 homologue) at a concentration of 100 ng/ml, and
- wherein the antibody has a cross-reactivity presented as B/B0 of greater than 80% against each one of the cross-reactants selected from the group consisting of (±)-CP-47,497 (C7 para quinone analogue), (±)-CP-55,940, (−)-CP 55,940, (+)-CP 55, 940, HU-210, HU-211 (dexanabinol), HU-308, delta9-THC, and (−)-11-nor-9-carboxy-delta9-THC, at a cross-reactant concentration of 100 ng/ml,
- wherein the B/B0 is measured using (±)-CP-47,497 (C8 homologue) as a standard and Tracer 1 as a tracer, of the following structural formula:
- wherein B0 is absorbance at 450 nm at zero ng/ml standard concentration, and
- wherein B is absorbance at 450 nm at predetermined variable standard concentrations.
5 more elements
Dependencies are inferred from the claim text.