Heat-transfer resistance based analysis of bioparticles

A bio-sensing device suitable for the detection and/or characterization of target bioparticles, the target bioparticles not being DNA bioparticles or RNA bioparticles, the bio-sensing device comprising:

• a heating element configured to heat using a power;
• a sample holder comprising a structured substrate having a surface comprising a plurality of binding cavities in which the target bioparticles can bind, the sample holder further being adapted for exposing the structured substrate at one side to the heating element;
• a first temperature sensor configured to sense a temperature at the side where the structured substrate can be exposed to the heating element and a second temperature sensor configured to sense a temperature at the side opposite thereto with respect to the structured substrate;
• a processing means programmed for calculating at least one heat transfer resistivity value based on temperature values obtained with the first temperature sensor and the second temperature sensor and the power for the heating element, the heat transfer resistivity value being defined as ΔT/P, in which P is the power of the heating element and ΔT is the difference between the temperature value of the first temperature sensor and the temperature value of the second temperature sensor, for deriving a characteristic of the target bioparticles from said heat transfer resistivity value.