Immunosorbent and immunoaffinity column for aflatoxin M1 nanobody and preparation method thereof

An aflatoxin M1 nanobody immunosorbent, comprising:

• a solid phase carrier;
• and
• an aflatoxin nanobody coupled to the solid phase carrier,
• wherein the aflatoxin nanobody consists of aflatoxin M1 nanobody 2014AFM-G2,
• wherein the aflatoxin M1 nanobody 2014AFM-G2 comprises the amino acid sequence of SEQ ID NO:7, encoded by the nucleic acid sequence of SEQ ID NO:8;
• wherein the aflatoxin M1 nanobody 2014AFM-G2 comprises further three complementary determining regions of:
• CDR1 consisting of amino acid sequence of SEQ ID NO:
• 1;
• CDR2 consisting of amino acid sequence of SEQ ID NO:2;
• and CDR3 consisting of amino acid sequence of SEQ ID NO:3;
• wherein the CDR1 is encoded by the nucleic acid sequence of SEQ ID NO:4;
• the CDR2 is encoded by the nucleic acid sequence of SEQ ID NO:5;
• and the CDR3 is encoded by the nucleic acid sequence of SEQ ID NO:6;
• and wherein the aflatoxin M1 nanobody immunosorbent specifically binds aflatoxin M1.