Optical analysis device, optical analysis method and computer program for optical analysis using single light-emitting particle detection

An optical analysis device which detects light from a light-emitting particle dispersed and moving at random in a sample solution using an optical system of a confocal microscope or a multiphoton microscope, comprising:

• a light detection region mover which moves a position of a light detection region of the optical system in the sample solution by changing an optical path of the optical system;
• a light detector which detects light from the light detection region;
• and
• a signal processor which generates time series light intensity data of the light from the light detection region detected with the light detector while moving the position of the light detection region in the sample solution and which detects a signal from each light-emitting particle individually in the time series light intensity data;
• wherein the signal processor detects the signal from each light-emitting particle by extracting a signal having a light intensity in a light intensity range having been set based upon a signal generation frequency integrated value distribution as the signal of the light-emitting particle from a group of signals detected in the time series light intensity data;
• wherein the signal generation frequency integrated value distribution is a distribution of integrated values of generation frequencies of signals having an intensity not lower than a variable;
• and
• wherein the variable is an intensity of a signal detected in the time series light intensity data.