Lab on chip cartridge

A method of manufacturing a cartridge for an optical process, comprising:

• forming a plurality of wells adapted to receive a sample to be analyzed in a supporting body having a first face and a second face opposite to one another, said wells extending in said supporting body on said first face;
• forming at least one biocompatible layer extending inside each well;
• forming an anti-reflection layer extending on said first face outside and exterior to said wells;
• and,
• forming a reflection layer extending inside each well, wherein together said biocompatible layer, said anti-reflection layer and said reflection layer define a passivation layer, wherein said passivation layer is composed of varying thicknesses.

A method of PCR analysis, said method comprising:

• providing a cartridge for an optical analysis, the cartridge including:
• a supporting body, having a first face and a second face opposite to one another;
• a plurality of wells adapted to receive a biological solution to be analyzed, said wells extending in said supporting body on said first face;
• at least one biocompatible layer extending inside each well;
• an anti-reflection layer extending on said first face outside and exterior to said wells;
• and,
• a reflection layer extending inside each well, wherein together said biocompatible layer, said anti-reflection layer and said reflection layer define a passivation layer, wherein said passivation layer is composed of varying thicknesses;
• adding nucleic-acid probes to each well of the cartridge;
• adding a solution containing two primers capable of binding to a target nucleic acid, nucleotides, nucleic-acid extender enzymes and a sample nucleic acid containing a target nucleic acid to each of said wells;
• performing a plurality of thermal cycles in said wells to amplify said target nucleic acid;
• and
• detecting hybridization of said amplified nucleic acid to said nucleic acid probes in each of said wells.