Method for concentrating sample constituents and for multiplying nucleic acids from a biological sample which are contained in the sample constituents

A method for concentrating sample constituents and for multiplying nucleic acids from a biological sample which are contained in the sample constituents, comprising:

• 1) providing a biological sample in liquid form;
• 2) introducing the sample into a separating system having at least one filter;
• 3) accumulating the sample constituents contained in the sample by separating off the sample constituents on the filter;
• 4) adding amplification reagents to the separating system;
• and
• 5) directly amplifying at least one nucleic acid present in the sample constituents;
• wherein the amplification in step 5) is carried out by PCR and comprises at least:
• 5.1) extracting nucleic acids without using chemical lysing agents and denaturing the at least one nucleic acid;
• 5.2) annealing primers;
• and
• 5.3) extending primers.