Production method for organic acid using CoA-transferase
AssigneesShowa Denko K.K., National University Corporation Hokkaido University
InventorsKen'ichiro Matsumoto, Seiichi Taguchi, Hirobumi Aoki
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Abstract
The present invention relates to a method of producing organic acids, comprising biochemical synthesis of organic acid-CoA using acetyl-CoA as a substrate and conducting CoA elimination reaction of the organic acid-CoA using CoA transferase in the presence of acetic acid, in which organic acid is obtained by culturing transformed microorganisms which have an enzyme gene cluster for the synthesis of organic acid-CoA using acetyl-CoA as a substrate and a CoA transferase gene.
Claims9
A method of producing hydroxycarboxylic acid having 3 to 6 carbon atoms, comprising biochemical synthesis of hydroxycarboxylic acid having 3 to 6 carbon atoms-CoA using acetyl-CoA as a substrate and conducting CoA elimination reaction of hydroxycarboxylic acid having 3 to carbon atoms-CoA using CoA transferase,
- in which hydroxycarboxylic acid having 3 to 6 carbon atoms is obtained by culturing transformed microorganisms which have enzyme genes that code for β-ketothiolase (PhaA), acetoacetyl-CoA reductase (PHaB), and propionic acid CoA transferase (PCT) in the presence of artificially added acetic acid and/or acetate in a medium.
A method of producing (R)-3-hydroxybutyryl-CoA, which comprises biochemical synthesis of (R)-3-hydroxybutyryl-CoA using acetyl-CoA as a substrate and conducting CoA elimination reaction of (R)-3-hydroxybutyryl-CoA using CoA transferase, in which (R)-3-hydroxybutyryl CoA is obtained by culturing transformed microorganisms which have enzyme genes that code for β-ketothiolase (PhaA), acetoacetyl-CoA reductase (PHaB), and propionic acid-CoA transferase (PCT) in the presence of artificially added acetic acid and/or acetate.
A method of producing (S)-3-hydroxybutyryl-CoA, which comprises biochemical synthesis of (S)-3-hydroxybutyryl-CoA using acetyl-CoA as a substrate and conducting CoA elimination reaction of (S)-3-hydroxybutyryl-CoA using CoA transferase, in which (S)-3-hydroxybutyryl CoA is obtained by culturing transformed microorganisms which have enzyme genes that code for β-ketothiolase (PhaA), acetoacetyl-CoA reductase (PHaB), and propionic acid-CoA transferase (PCT) in the presence of artificially added acetic acid and/or acetate.
Dependencies are inferred from the claim text.