Proteins relating to grain shape and leaf shape of rice, coding genes and uses thereof
AssigneeInstitute of Subtropical Agriculture, Chinese Acad
InventorXinjie Xia
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Abstract
The present invention provides a protein OsXCL relating to grain shape and leaf shape of rice, its derived proteins and their coding genes. Transgenic rices over-expressing OsXCL gene present phenotypes as increase of grain length, grain weight and number of grains per panicle, and leaf rolling and so on. The present invention also provides the method for obtaining transgenic plants by transforming the coding genes of OsXCL or its derived proteins into objective plants.
Claims9
A method for increasing one or more of grain weight, grain length, grain number per panicle and/or rolled leaves of a plant, which comprises:
- introducing a recombinant expression vector comprising a nucleic acid encoding the protein as set forth by SEQ ID NO. 2 into a target plant;
- screening the transgenic plant for the recombinant expression vector;
- and
- breeding the transgenic plant wherein the plant exhibits increased grain weight, increased grain length, increased grain number per panicle and/or rolled leaves compared to a non-transgenic plant.
A method for increasing one or more of grain weight, grain length, grain number per panicle and/or rolled leaves of a plant, comprising:
- regenerating a transgenic cell line or breeding a recombinant plant with another plant in a selection medium to obtain a transgenic plant, wherein the cell line or recombinant plant comprises a heterologous nucleic acid selected from
- a) a nucleic acid encoding the polypeptide that is encoded by nucleotides 106-870 of SEQ ID NO. 1;
- b) a nucleic acid encoding the polypeptide that is encoded by nucleotides 50-873 of SEQ ID NO. 1;
- and
- selecting and growing a plant with increased grain weight, increased grain length, increased grain number per panicle and/or rolled leaves of the transgenic plant with compared to a non-transgenic plant.
A method for increasing one or more of grain weight, grain length, grain number per panicle and/or rolled leaves of a plant, which comprises:
- introducing a recombinant expression vector comprising a nucleic acid selected from:
- a) a nucleic acid encoding the polypeptide that is encoded by positions 106-870 from the 5′ end of SEQ ID NO. 1;
- b) a nucleic acid encoding the polypeptide that is encoded by positions 50-873 from the 5′ end of SEQ ID NO. 1;
- into a target plant;
- screening the transgenic plant for the recombinant expression vector;
- and identifying and breeding the transgenic plant wherein the plant exhibits increased grain weight, increased grain length, increased grain number per panicle and/or rolled leaves compared to a non-transgenic plant.
Dependencies are inferred from the claim text.