Method for removing cells
AssigneeNational Institute of Advanced Industrial Science and Technology
InventorsKimio Sumaru, Toshiyuki Takagi, Kyoko Kikuchi, Taku Sato, Manae Yamaguchi, Toshiyuki Kanamori
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Abstract
A method for separating cells which includes: adhering cells to the surface of a cell culture substrate containing a photo-acid generator that generates an acidic substance upon irradiation with active energy rays, and irradiating only a partial region of the cell culture substrate with the active energy rays to selectively remove the cells within the partial region, thereby separating the cells within the partial region and cells in other regions.
Claims12
A method for removing target cells from a cell culture substrate, the method comprising:
- providing a cell culture substrate including a substrate and a layer containing a photo-acid generator formed on a surface of the substrate:
- adhering cells to the surface of the layer containing the photo-acid generator which generates an acidic substance upon irradiation with light,
- generating the acidic substance by irradiating only a region of the substrate containing the photo-acid generator layer beneath target cells to be removed, and
- removing the target cells from the region of the irradiated substrate by the action of the acidic substance generated from the irradiated layer containing the photo-acid generator beneath the target cells.
Dependencies are inferred from the claim text.