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US 9,434,934 B2Granted
Heat stable keratinase and use thereof
AssigneeAcademia Sinica
InventorsMeng-Chiao Ho, Shih-Hsiung Wu, Wan-Ling Wu
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AssigneeAcademia Sinica
InventorsMeng-Chiao Ho, Shih-Hsiung Wu, Wan-Ling Wu
Estimating the term…
A fusion gene encoding M. taiwanensis WR-220 keratinase is disclosed. The fusion comprises: (a) a first DNA sequence encoding a protein secretion signal peptide, located at the N-terminus of the fusion gene; (b) a second DNA sequence encoding an inhibitory domain of M. taiwanensis WR-220 keratinase, linked in translation frame with the first DNA sequence; and (c) a third DNA sequence encoding a catalytic domain of M. taiwanensis WR-220 keratinase, linked in translation frame with the second DNA sequence, wherein the fusion gene is a non-naturally occurring chimeric DNA. Also disclosed are a method for preparation of the catalytic domain of M. taiwanensis WR-220 keratinase, and use of the M. taiwanensis WR-220 keratinase.
A fusion gene comprising:
An isolated protease comprising a catalytic domain of M. taiwanensis WR-220 keratinase, the protease lacking an inhibitory domain of M. taiwanensis WR-220 keratinase, and being in a dosage form of tablet, caplet, pellet, capsule, granule, pill, powder or sachet, or in a dosage form of solution without containing a cell culture supplement.
Dependencies are inferred from the claim text.