US 9,434,932 B2Granted

Alpha-amylase variants

AssigneeNovozymes A/S

InventorsSvend Kaasgaard, Jens Oebro, Signe Eskildsen Larsen, Allan Svendsen, Annette Helle Johansen, Michael Skjoet, Carsten Andersen, Lars Beier, Esben Peter Friis, Miguel Duarte Guilherme Pereira Toscano, Mads Bjoernvad, Frank Winther Rasmussen, Liv Spaanger Christiansen

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Abstract

The present invention relates to variants of a parent alpha-amylase. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

Claims21

Dependent claims

An isolated variant of a parent alpha-amylase, comprising at least two substitutions at positions corresponding to 140 and 206 of the polypeptide of SEQ ID NO:

1, wherein the variant has at least 90% sequence identity with the polypeptide of SEQ ID NO:
1, and has alpha-amylase activity, and wherein the substitution at position 206 is V206Y.

Dependencies are inferred from the claim text.

Facts

Application: 14/129,565Filed 29 Jun 2012 · us provisional application
Priority: 1 Jul 2011
Published: US 2014/0141489 A122 May 2014
Granted: 6 Sep 2016
Term ends: Not estimatedThe source records don’t support an estimate.

Classification6 codes

C12N 9/2417
Alpha-amylase: from microbiological source
C12N 9/2414
Amylases: alpha-amylase
C12N 15/63
Recombinant DNA-technology: introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
C12Y 302/01001
Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds: alpha-amylase

Family

Application61/503,768Us provisional application · filed 1 Jul 2011
US 2014/0141489 A1Pre-grant publication · published 22 May 2014
US 9,434,932 B2This patent

From the US filing history. Foreign family members aren’t listed.

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