Method for manufacturing cell culture substrate

A method for manufacturing a cell culture substrate comprising a substrate and a coating layer of a polymer formed on the surface of the substrate, said method comprising the steps of,

• polymerizing a monomer component comprising a nitrogen atom-containing monomer represented by the formula (I) on the surface of a substrate having hydroxyl groups thereon, to form a coating layer of the polymer on said surface:
• wherein R1 is hydrogen atom or methyl group, R2 is an alkylene group having 1 to 6 carbon atoms, each of R3 and R4 is independently an alkyl group having 1 to 4 carbon atoms, R5 is an alkylene group having 1 to 4 carbon atoms, and Y is oxygen atom or —NH— group, and
• irradiating the coating layer of the polymer at a predetermined position with ion beam, to remove the coating layer of the polymer irradiated with the ion beam at the predetermined position.