Plants having stress related traits and methods for making the same
AssigneeDow AgroSciences LLC
InventorsAvutu S. Reddy, Sun Yuejin, Donald J. Merlo, Weiting W. Ni, Dayakar Pareddy, Zhifang Gao, Gregory A. Bradfisch
Life of this patent
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Abstract
This invention relates generally to a plant cell with increased yield, preferably under condition of transient and repetitive abiotic stress as compared to a corresponding non-transformed wild type plant cell by increasing or generating one or more activities of RING proteins in plants.
Claims22
A transgenic monocotyledous plant comprising in its genome a stably-integrated, recombinant DNA construct comprising a polynucleotide operably linked to at least one regulatory element, wherein said polynucleotide has a nucleic acid sequence encoding SEQ ID NO:
- 2, and wherein said monocotyledous plant exhibits increased drought tolerance when compared to a control plant not comprising said recombinant DNA construct.
A method of increasing drought and/or heat tolerance in a monocotyledous plant, comprising:
- (a) introducing into a regenerable plant cell a recombinant DNA construct comprising a polynucleotide operably linked to at least one regulatory sequence, wherein the polynucleotide has a nucleic acid sequence encoding SEQ ID NO:
- 2;
- and (b) regenerating a transgenic plant from the regenerable plant cell after step (a), wherein the transgenic plant comprises stably-integrated in its genome the recombinant DNA construct and the transgenic plant exhibits increased drought tolerance when compared to a control plant not comprising the recombinant DNA construct.
A method of improving drought tolerance in a corn line comprising crossing a corn plant of said corn line with a drought tolerant transgenic corn plant having a recombinant DNA expressing a protein having the amino acid sequence of SEQ ID NO:2 to produce a corn seed having the recombinant DNA.
A method for increasing yield in a corn crop subject to water deficit during its growth, said method comprising planting transgenic seeds having a stably-integrated, recombinant DNA, wherein the stably-integrated, recombinant DNA comprises the nucleic amino acid sequence of SEQ ID NO:1 or SEQ ID NO:
- 3 or wherein the stably-integrated, recombinant DNA encodes a protein having the amino acid sequence of SEQ ID NO:2 and allowing said seeds to grow to mature corn plants while being subjected to drought during at least one ten day period during vegetative phase of growth.
A drought tolerant transgenic corn plant having a recombinant DNA expressing a protein having the amino acid sequence of SEQ ID NO:2.
Transgenic seed for growing a drought tolerant corn plant, said seed comprising in its genome a stably-integrated, recombinant DNA encoding a protein having the amino acid sequence of SEQ ID NO:
- 2.
A DNA construct comprising a nucleic acid encoding the polypeptide of SEQ ID NO:
- 2, wherein the nucleic acid is operably linked to a heterologous regulatory element and wherein SEQ ID NO:
- 2 increases the maintenance of membrane integrity under abiotic stress in a plant cell.
A method for making a transgenic monocotyledous plant having an drought related trait relative to a control plant, comprising introducing a recombinant construct into a plant cell, plant, or part thereof, wherein the drought related trait is one or more of:
- (i) increased early vigor;
- (ii) increased biomass;
- (iii) increased total seed yield per plant;
- (iv) increased seed filling rate;
- (v) increased harvest index;
- (vi) increased thousand kernel weight, (vii) increased abiotic stress resistance, or (viii) increased nutrient uptake efficiency, and wherein the construct comprises (a) a nucleic acid sequence encoding the polypeptide of SEQ ID NO:
- 2;
- (b) one or more control sequences capable of driving expression of the nucleic acid sequence of (a) and optionally (c) a transcription termination sequence.
Dependencies are inferred from the claim text.