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US 9,434,771 B2Granted
Compositions and methods for diagnosing and treating inflammatory bowel disease and related disorders
AssigneeThe Johns Hopkins University
InventorsXuhang Li, Heng Zhu
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Abstract
The present invention features biomarkers capable of diagnosing inflammatory bowel disease and methods of using such biomarkers to diagnose and selecting treatments for inflammatory bowel diseases.
Claims11
A method for differentiating ulcerative colitis from Crohn's disease or vice versa in a human subject, the method comprising
- (a) obtaining a blood, serum or plasma sample from the human subject,
- (b) contacting the blood, serum or plasma sample of the human subject with an E. coli frvX polypeptide, thereby forming a human antibody-frvX polypeptide complex,
- (c) contacting the blood, serum or plasma sample of the human subject with an E. coli yidX polypeptide, thereby forming a human antibody-yidX polypeptide complex,
- (d) contacting the human antibody-frvX polypeptide complex and the human antibody-yidX polypeptide complex with a Cy3-labeled donkey anti-human antibody, thereby forming a first complex comprising Cy3-labeled donkey anti-human antibody-human antibody-frvX polypeptide and a second complex comprising Cy3-labeled donkey anti-human antibody-human antibody-yidX polypeptide;
- and
- (e) detecting the first complex and the second complex, wherein detection of a greater amount of the first complex than the second complex, or detection of an amount of the first complex equal to the second complex, identifies the subject as having ulcerative colitis, wherein detection of a greater amount of the second complex than the first complex identifies the subject as having Crohn's disease,
- thereby differentiating ulcerative colitis from Crohn's disease, or vice versa, in the human subject.
4 more elements
Dependencies are inferred from the claim text.