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US 9,433,947 B2Granted
Centrifugal microfluidic disk and processing method using the same
AssigneeNational Taiwan University
InventorsAndrew Man Chung Wo, Chen-Lin Chen, Cheng-Wei Yang, Wei-Hao Lian
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Abstract
This invention provides a centrifugal microfluidic disk and methods for separating targets or cells and collecting the targets or cells in the centrifugal microfluidic disk by using the density gradient.
Claims19
A centrifugal microfluidic disk, at least comprising:
- a sample inlet, located at a first level for loading a blood or biological sample;
- a separation chamber, connected to the sample inlet, wherein the separation chamber contains an arc-shape portion and a density gradient solution is loaded into the separation chamber;
- a chemical inlet, located at a second level for loading a chemical;
- a collection chamber, connected to the separation chamber;
- a conveying channel, connected to the collection chamber;
- and
- a junction extending vertically from the first level to the second level and connecting the conveying channel located at the second level and the separation chamber located at the first level, wherein the chemical inlet and the sample inlet are located at different levels.
4 more elements
A centrifugal microfluidic disk, at least comprising:
- a sample inlet, located at a first level for loading a blood or biological sample;
- a separation chamber, connected to the sample inlet, wherein the separation chamber contains an arc-shape portion and a density gradient solution is loaded into the separation chamber;
- and
- a settling chamber, connected to the separation chamber;
- a chemical inlet, located at a second level for loading a chemical;
- a collection chamber, connected to the separation chamber;
- a conveying channel, connected to the collection chamber;
- and
- a junction extending vertically from the first level to the second level and connecting the conveying channel located at the second level and the separation chamber located at the first level, wherein the chemical inlet is connected to the collection chamber via a linking channel and a first flow path of the sample is different than a second flow path of the chemical, and the chemical inlet and the sample inlet are located at different levels.
6 more elements
A method for separating a target from a sample, comprising:
- providing a microfluidic disk, wherein the microfluidic disk at least comprises:
- a sample inlet located at a first level for loading a blood or biological sample;
- a separation chamber, connected to the sample inlet, wherein the separation chamber contains an arc-shape portion;
- a chemical inlet, located at a second level;
- a collection chamber, connected to the separation chamber through at least a flow channel located in-between;
- a conveying channel, connected to the collection chamber;
- and
- a junction extending vertically from the first level to a second level and connecting the conveying channel located at the second level and the separation chamber located at the first level, wherein the first and second levels are different levels;
- introducing the sample into the sample inlet of the microfluidic disk and loading a density solution into the separation chamber;
- and
- spinning the microfluidic disk to drive the sample flowing outward through the separation chamber, wherein the target is separated from the sample by the density solution in the separation chamber and further flows through the junction and the conveying channel into the collection chamber.
8 more elements
Dependencies are inferred from the claim text.