Back to results
US 9,433,646 B2Granted
Methods of inhibiting growth of a neoplastic cell
AssigneeXON Cells, Inc.
InventorThomas E. Ichim
Life of this patent
Estimating the term…
Abstract
Disclosed are methods of preparing and using placentally-derived stem cells and compositions useful for the treatment of cancer. Said stem cells and compositions function through inducing a “guided differentiation” program in cancer cells, thereby reducing malignancy. Further extension of the invention pertains to augmenting ability of administered cells to induce differentiation through the co-administration of known differentiation inducing agents. Within the context of this disclosure, methods for inducing host responses to cancer are also described.
Claims7
A method of inhibiting growth of a neoplastic cell, the method comprising:
- (a) providing a cell free-placental extract made by the process comprising:
- (i) obtaining human placenta;
- (ii) homogenizing the obtained human placenta by digesting the obtained human placenta with a collagenase and a heparitinase, wherein the heparitinase digestion is at a neutral pH, the homogenization resulting in a homogenate;
- (iii) centrifuging said homogenate to produce a supernatant free of cellular debris;
- and
- (iv) purifying the supernatant to obtain cell free-placental extract comprising a cell differentiation-inducing fraction having a molecular weight in the range from about 10 Daltons to about 10,000 Daltons
- (b) directly administering said cell free-placental extract to the neoplastic cell which is selected from the group consisting of a leukemia cell, and a prostate cancer cell, thereby inhibiting growth of the neoplastic cell.
4 more elements
Dependencies are inferred from the claim text.