Multilayered implant materials derived from amniotic membrane, methods of making the multilayered implant materials, and method of using multilayered implant materials
AssigneeUniversity of Florida Research Foundation, Inc.
InventorPeter McFetridge
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Abstract
Exemplary embodiments of the present disclosure provide for multi-layered implantation materials, methods of making a multi-layered implantation material, methods of forming a multi-layered implantation material, methods of forming a multi-layered implantation material having a spiral roll cross-section, and the like.
Claims30
A method of forming a multi-layered implantation material, comprising the steps of:
- obtaining an amniotic membrane;
- decellularizing the amniotic membrane to produce a decellularized amniotic membrane;
- forming a multi-layered implantation material by wrapping the amniotic membrane around itself to form a multi-layered structure having a spiral roll cross-section with two or more spiral layers;
- freeze drying the multi-layered implantation material;
- and
- after forming the multi-layered implantation material, culturing the multi-layered implantation material under conditions whereby the membrane is colonized by a population of cells.
A method of forming a multi-layered implantation material having a spiral roll cross-section, comprising the steps of:
- wrapping an amniotic membrane around itself to form a multi-layered structure having the spiral roll cross-section, wherein the multi-layered implant has two or more spiral layers;
- and
- freeze drying the multi-layered structure.
A method of forming a non-synthetic, multi-layered implantation tubular scaffold, the method comprising:
- wrapping an amniotic membrane around itself to form a multi-layered implantation tubular scaffold having a spiral roll cross-section with two or more spiral layers;
- optionally freeze drying the multi-layered implantation tubular scaffold;
- and
- optionally culturing the multi-layered implantation tubular scaffold under conditions whereby the membrane is colonized by a populations of cells;
- wherein the multi-layered implantation tubular scaffold does not comprise synthetic materials.
Dependencies are inferred from the claim text.