System and method for quantifying fragile X mental retardiation 1 protein in tissue and blood samples

An assay system for detecting a fragile X mental retardation protein in a human tissue sample, comprising:

• a monoclonal antibody that binds to a first epitope of the human fragile X mental retardation protein;
• a polyclonal antibody that binds to a second, different epitope of the fragile X mental retardation protein;
• and
• a third antibody that binds to said polyclonal antibody and is conjugated to a fluorescent compound;
• wherein the monoclonal antibody comprises a monoclonal mouse antibody that binds to SEQ. ID NO. 3, the polyclonal antibody comprises a polyclonal rabbit antibody that binds to SEQ. ID NO. 1, and the third antibody is an anti-rabbit immunoglobulin G antibody.