Liposome, proteoliposome, biochip, and method for producing liposome and proteoliposome

A method for producing a proteoliposome having a region of a lipid bilayer membrane with different membrane thicknesses comprising:

• a mixing step to obtain a mixed solution by mixing two or more kinds of lipids whose phase transition temperatures are different, a surfactant and a membrane protein;
• a temperature adjusting step to adjust the temperature of the mixed solution to be between the highest phase transition temperature among phase transition temperatures of the lipids and the lowest phase transition temperature among phase transition temperatures of the lipids;
• a membrane forming step to form a lipid bilayer membrane by removing the surfactant from the mixed solution after the temperature adjusting step;
• and
• an ultrasonic agitating step to agitate the lipid bilayer membrane by exposing the lipid bilayer membrane to an ultrasonic wave,
• wherein the lipids are free of cholesterol.