Compositions and methods for capture and elution of biological materials via particulates

A method of capturing a biological material from a fluid specimen and lysing the fluid specimen, the method comprising:

• introducing the fluid specimen containing the biological material into a flow-through device comprising a first chamber, the first chamber containing a solid phase material retained in the first chamber, the solid phase material having an affinity for the biological material;
• the first chamber having an inlet opening and an outlet opening, the inlet opening and the outlet opening allowing flow of the fluid specimen into and out of the first chamber, the first chamber further having a first fitting attached to the inlet opening of the first chamber, the first fitting having a first thin filter insert that retains the solid phase material while allowing rapid flow of the fluid specimen at low pressure, the first chamber further having a second fitting attached to the outlet opening of the first chamber, the second fitting having a second thin filter insert that retains the solid phase material while allowing rapid flow of the fluid specimen at low pressure;
• flowing the fluid specimen containing the biological material through the solid phase material, the fluid specimen exiting the first chamber via the outlet opening;
• and lysing the fluid specimen in a second chamber fluidly connected to the first chamber, the second chamber comprising:
• an impeller which in use imparts rotational motion to the fluid specimen to mechanically lyse the fluid specimen.