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US 9,427,737 B2Granted
Methods and compositions for using oils for analysis and detection of molecules
AssigneeBio-Rad Laboratories, Inc.
InventorsNicholas Jack Heredia, Anthony J. Makarewicz, Jr.
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Abstract
Oil compositions and methods for use in droplet formation or in spacing of droplets are disclosed. The oil compositions may include a combination, such as a silicone oil and a fluorine-containing compound. The droplets can contain analytes (e.g., nucleic acids) and may be used for molecular reactions (e.g., digital PCR) and detection.
Claims19
A composition comprising:
- aqueous droplets;
- and
- a homogeneous oil mixture forming a continuous phase that encapsulates each of the aqueous droplets, the oil mixture including (a) a silicone oil and (b) a fluorinated oil;
- wherein the concentration of said silicone oil in the oil mixture is at least about 50% by weight, and
- wherein the aqueous droplets have a neutral buoyancy in the oil mixture.
2 more elements
A composition comprising:
- aqueous droplets;
- a homogeneous oil mixture including (a) a silicone oil and (b) a fluorinated oil, wherein the concentration of said silicone oil in the oil mixture is at least about 50% by weight;
- and
- a continuous oil phase encapsulating a dispersed first portion of the oil mixture and contacting but not encapsulating a continuous second portion of the oil mixture;
- wherein each of the dispersed first portion and the continuous second portion of the oil mixture encapsulates a plurality of the aqueous droplets.
2 more elements
Dependencies are inferred from the claim text.