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US 9,040,770 B2Granted
Modalities for the treatment of degenerative diseases of the retina
AssigneeOcata Therapeutics, Inc.
InventorsIrina Klimanskaya, Robert Lanza
Life of this patent
Estimating the term…
Abstract
This invention relates to methods for improved cell-based therapies for retinal degeneration and for differentiating human embryonic stem cells and human embryo-derived into retinal pigment epithelium (RPE) cells and other retinal progenitor cells.
Claims16
A method of producing isolated human RPE cells, comprising:
- (a) culturing a multilayer culture of human pluripotent cells that express Oct-4, alkaline phosphatase, SSEA-3, SSEA-4, TRA-I-60, and TRA-I-81 under adherent conditions for a sufficient time for the appearance of a pigmented cell population having a cobblestone, polygonal, epithelial-like appearance and comprising cells that express RPE65 and bestrophin;
- and
- (b) isolating the pigmented cell population from the resultant cell culture, thereby obtaining human RPE cells.
A method of producing isolated human RPE cells, comprising:
- (a) culturing embryoid bodies comprising human pluripotent cells that express Oct-4, alkaline phosphatase, SSEA-3, SSEA-4, TRA-I-60, and TRA-I-81for a sufficient time for the appearance of a pigmented cell population having a cobblestone polygonal epithelial-like appearance and comprising cells that express RPE65 and bestrophin;
- and
- (b) isolating the pigmented cell population from the resultant cell culture, thereby obtaining human RPE cells.
A method of producing isolated human RPE cells, comprising:
- (a) differentiating in vitro human pluripotent cells that express Oct-4, alkaline phosphatase,SSEA-3, SSEA-4, TRA-I-60, and TRA-I-81to obtain a culture of a pigmented cell population having a cobblestone polygonal epithelial-like appearance and comprising cells that express RPE65 and bestrophin;
- and
- (b) isolating the pigmented cell population from the resultant cell culture, thereby obtaining human RPE cells.
Dependencies are inferred from the claim text.