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US 9,040,694 B1Granted
Compounds and methods useful for directing stem cell differentiation
AssigneeVanderbilt University
InventorsCharles C. Hong, Corey R. Hopkins, Antonis K. Hatzopoulos, Craig W. Lindsley, Jijun Hao
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Abstract
The presently-disclosed subject matter relates to compounds of the formula: and methods for use thereof. The presently-disclosed subject matter relates methods of selectively differentiating a stem cell, and methods of screening for compounds useful for enhancing terminal differentiation of committed cardiac progenitor cells.
Claims16
A compound of the formula:
- wherein R1 is selected from the group consisting of
A method of selectively differentiating a stem cell, comprising:
- providing the stem cell;
- and
- contacting the stem cell with an effective amount of a compound of the formula,
- wherein R1 is selected from the group consisting of
- and
- wherein the stem cell becomes differentiated into a cardiomyocyte.
3 more elements
A method of screening for a compound useful for enhancing terminal differentiation of committed cardiac progenitor cells, comprising:
- providing a first pluripotent stem cell and a second pluripotent stem cell;
- contacting the first and second pluripotent stem cells with an effective amount of a first compound of the formula,
- wherein R1 is selected from the group consisting of
- contacting the second cells with a test compound;
- identifying at least one indicator of cardiomyogenesis exhibited by the first cells and the second cells;
- comparing the appearance of the at least one indicator of cardiomyogenesis exhibited by the first cells to the appearance of the at least one indicator of cardiomyogenesis exhibited by the second cells;
- and
- identifying the test compound as a compound useful for enhancing terminal differentiation of committed cardiac progenitor cells if the appearance of the at least one indicator of cardiomyogenesis in the second cells is accelerated and/or augmented as compared to the appearance of the at least one indicator of cardiomyogenesis in the first cells.
5 more elements
Dependencies are inferred from the claim text.