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US 9,040,675 B2Granted
Formulations for nucleic acid stabilization on solid substrates
AssigneeGeneral Electric Company
InventorsBrian Christopher Bales, Erik Leeming Kvam, Jason Louis Davis
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Abstract
The present disclosure generally relates to dry solid matrices for the extraction, stabilization, and storage of nucleic acids, particularly RNA, in a dry format under ambient conditions for a prolonged period of time. Methods for collecting and recovering the nucleic acids stored in the dry solid matrix are also described.
Claims23
A dry solid matrix for extraction and storage of nucleic acids from a biological sample, wherein a composition comprising;
- a metal thiocyanate comprises a Group 1 or Group 2 metal cation;
- a reducing agent where the reducing agent is tris(2-carboxyethyl)phosphine (TCEP);
- and
- a buffer;
- is incorporated into the dry solid matrix, and the matrix is then dried;
- and where the solid matrix is a porous matrix comprising cellulose, cellulose acetate, glass fiber, or any combination thereof.
3 more elements
A method for extracting and storing nucleic acids from a sample comprising:
- a) providing a dry solid matrix for extraction and storage of nucleic acids from a biological sample, where the solid matrix is a porous matrix comprising cellulose, cellulose acetate, glass fiber, or any combination thereof, and wherein a composition is incorporated into the dry solid matrix comprising,
- a metal thiocyanate comprises a Group 1 or Group 2 metal cation;
- a reducing agent ,where the reducing agent is TCEP;
- and a buffer;
- b) drying the solid matrix after incorporation of the composition into the dry solid matrix;
- c) applying a sample to the dry solid matrix to collect the nucleic acids;
- d) drying the solid matrix comprising the nucleic acids;
- and
- e) storing the nucleic acids on the solid matrix in a dry state under ambient conditions.
6 more elements
Dependencies are inferred from the claim text.