Compositions, methods and kits based on small nuclear RNAs
AssigneeThe Trustees of the University of Pennsylvania
InventorsGideon Dreyfuss, Jeongsik Yong, Tracey (Golembe) Polsky
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Abstract
The present invention includes methods for modulating the expression of a gene or mRNA in a cell using small nuclear RNAs comprising specific sequence and structural features and further comprising a nucleic acid cargo.
Claims8
A composition comprising a nucleic acid cargo sequence and an isolated snRNA, wherein said cargo sequence is linked to said snRNA, further wherein said snRNA consists of:
- (a) an Sm site, wherein said Sm site is selected from the group consisting of SEQ ID NO. 1, SEQ ID NO. 2, and SEQ ID NO. 3;
- (b) a stem-loop structure positioned 3′ to said Sm site, wherein the number of nucleotides between the Sm site and the 3′ stem loop is between 1 and 5 nucleotides, further wherein said stem-loop structure comprises from about 7 to about 12 base pairs in the stem and from about 4 to about 17 nucleotides in the loop;
- and
- (c) nucleotides located 3′ from the stem-loop structure consisting of 5-10 nucleotides.
A kit for modulating the expression of a gene in a cell, said kit comprising a composition comprising a nucleic acid cargo sequence and an isolated snRNA, wherein said cargo sequence is linked to said snRNA, further wherein said snRNA consists of:
- (a) an Sm site, wherein said Sm site is selected from the group consisting of SEQ ID NO. 1, SEQ ID NO. 2, and SEQ ID NO. 3;
- (b) a stem-loop structure positioned 3′ to said Sm site, wherein the number of nucleotides between the Sm site and the 3′ stem loop is between 1 and 5 nucleotides, further wherein said stem-loop structure comprises from about 7 to about 12 base pairs in the stem and from about 4 to about 17 nucleotides in the loop;
- and
- (c) nucleotides located 3′ from the stem-loop structure consisting of 5-10 nucleotides;
- wherein, said kit further comprising an applicator and an instructional material for the use thereof.
An isolated molecule consisting of a nucleic acid cargo sequence and an isolated snRNA, wherein said snRNA:
- (a) an Sm site, wherein said Sm site is selected from the group consisting of SEQ ID NO. 1, SEQ ID NO. 2, and SEQ ID NO. 3;
- (b) a stem-loop structure positioned 3′ to said Sm site, wherein the number of nucleotides between the Sm site and the 3′ stem loop is between 1 and 5 nucleotides, further wherein said stem-loop structure comprises from about 7 to about 12 base pairs in the stem and from about 4 to about 17 nucleotides in the loop;
- and
- (c) nucleotides located 3′ from the stem-loop structure consisting of 5-10 nucleotides.
Dependencies are inferred from the claim text.