US 9,040,484 B2 · Production and delivery of a stable collagen

Abstract

Improved methods are provided for the recombinant synthesis of collagen, particularly collagen VII, in host cell, and for therapeutic delivery of the same. The recombinant collagen is produced in a host cell that has increased levels of prolyl-4-hydroxylase, relative to basal cell levels. The collagen produced by the methods of the invention has increased numbers of modified proline residues, relative to a recombinant collagen produced in a host cell having basal levels of prolyl-4-hydroxylase. The increased proline modification provides for a collagen having increased stability, including increased in vivo stability.

Claims9

Dependent claims

A method of synthesizing collagen VII, the method comprising:

synthesizing recombinant collagen VII in a host cell that has been genetically modified to increase expression of prolyl-4-hydroxylase, wherein the genetic modification to increase expression of prolyl-4-hydroxylase comprises (i) introduction into the cell of an episomal vector comprising a genetic sequence encoding prolyl-4-hydroxylase operably linked to a promoter, or (ii) modifying the genomic sequence of an endogenous prolyl-4-hydroxylase in said cell to increase expression;
wherein recombinantly produced collagen VII has increased stability relative to a collagen produced in a comparable host cell lacking said genetic modification.

Dependencies are inferred from the claim text.

Facts

Application

14/112,444Filed 26 Apr 2012 · us provisional application

Priority

26 Apr 2011

Published

US 2014/0107036 A117 Apr 2014

Granted

26 May 2015

Term ends

Not estimatedThe source records don’t support an estimate.

Classification10 codes

C07K 14/78
From animals; from humans: connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
A61K 38/39
From animals; from humans: connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
A61M 37/0015
Other apparatus for introducing media into the body ; Percutany, i.e. introducing medicines into the body by diffusion through the skin: by using…
C12N 9/0071
Oxidoreductases: acting on paired donors with incorporation of molecular oxygen

Production and delivery of a stable collagen

Life of this patent

Production and delivery of a stable collagen

Life of this patent

Abstract

Claims9