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US 9,040,298 B2Granted
Method of selecting stem cells having high chondrogenic differentiation capability
AssigneeMedipost Co., Ltd.
InventorsYoon-Sun Yang, Won Il Oh, Hong Bae Jeon, Mee Hyun Jung, Sang Young Jeong
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Abstract
Thrombospondin 1 (TSP-1), TSP-2, interleukin 17B receptor (IL-17BR) and heparin-binding epidermal growth factor-like growth factor (HB-EGF) associated with stem cell activity and use thereof.
Claims5
A method of selecting a stem cell capable of differentiating into a chondrocyte or inducing chondrocyte differentiation, the method comprising:
- culturing a stem cell in a monolayer or pellet in a maintenance medium;
- measuring a concentration of TSP-2;
- and
- selecting and isolating a stem cell having a high chondrogenic differentiation capability from the culture medium if
- a) the concentration of TSP-2 in cell culture is larger than 72 pg/ml/1.0×105 cells when the stem cell is monolayer cultured for 1 day, or
- b) the concentration of TSP-2 in cell culture is larger than 550 pg/ml/1.0×105 cells when the stem cell is pellet cultured for 7 days,
- wherein the selected stem cell is capable of differentiating into a chondrocyte or inducing chondrocyte differentiation.
4 more elements
Dependencies are inferred from the claim text.