A mannanase variant having mannanase activity and an amino acid sequence that varies from the amino acid sequence of parent/wild type Trichoderma reesei mannanase (SEQ ID NO:1, wherein the amino acid sequence of the mannanase variant comprises the variations 3R, 201S, 207F and 274L, and further variations selected from the group consisting of:
- 1) F31Y/S66P/Q97R/N113Y/N173H/V181H/A215T/Q259R/Q280R/N282D/N331S;
- 2) S66P/Q97R/N113Y/N173H/V181A/A215T/Q259R/Q280S/N331S;
- 3) F31Y/S66P/Q97R/N173H/V181H/A215TIQ259R/Q280R/N282D;
- 4) F31Y/S66P/Q97R/N173H/V181H/A215T/Q259R/Q280S/N282D/N331S;
- 5) F31Y/S66P/Q97R/N113Y/N173H/V181H/A215T/Q259R/Q280R/N282D;
- 6) F31Y/S66P/Q97R/Q149K/N173H/V181H/A215T/Q259R/Q280L;
- 7) S66P/Q97R/N113Y/N173H/V181A/A215T/Q259R;
- 8) F31Y/S66P/Q97R/N113Y/N173H/V181H/A215T/Q259R/Q280S/N282D/N331S;
- 9) S66P/N113Y/N173H/V181H/A215T/Q259R;
- 10) F31Y/S66P/Q97R/N113Y/K146Q/N173H/V181H/A215T/Q259R/Q280L/N28 2D;
- 11) F31Y/S66P/Q97R/N113Y/K146Q/N173H/V181A/A215T/Q259R/Q280L/N28 2D/ N331S;
- 12) F31Y/S66P/Q97R/N113Y/K146Q/N173H/V181H/A215T/Q259R/Q280L;
- 13) F31Y/S66P/Q97R/N173H/V181H/A215T/Q259R/N282D;
- 14) F31Y/S66P/Q97R/N113Y/K146Q/N173H/V181A/A215T/Q259R/Q280R/N28 2D;
- 15) S66P/N113Y/V181H/A215T/Q259R;
- 16) S66P/Q97R/N113Y/N173H/V181H/A215T/Q259R/Q280L/N282D;
- 17) F317/S66P/Q97R/N113YNK146Q/N173H/V181A/A215T/Q259R/Q280L/N28 2D;
- 18) F31Y/S66P/N173H/V181H/A215T/Q259R/N282D;
- 19) F31Y/S66P/Q97R/N173H/V181H/A215T/Q259R/Q280L;
- 20) S66P/Q97R/N113Y/N173H/V181H/A215T/Q259R/Q280R/N282D;
- 21) F31Y/S66P/Q97R/N113Y/N173T/V181H/A215T/Q259R/Q280R/N282D;
- 22) F31Y/S66P/Q97R/N173T/V181H/A215T/Q259R/Q280R/N282D;
- 23) F31Y/S66P/Q97R/N173H/V181H/A215T/Q259R/Q280S/N282D;
- 24) S66P/N113Y/N173H/V181H/A215T/Q259R/Q280S/N282D;
- 25) S66P/Q97R/N113Y/N173H/V181A/A215T/Q259R/Q280S/N282D/N331S;
- 26) S66P/Q97R/N113Y/V181H/A215T/Q259R/Q280L/N282D;
- 27) S66P/N113Y/N173H/V181H/A215T/Q259R/N331S;
- 28) F31Y/S66P/Q97R/N113Y/K146Q/N173H/V181A/A215T/Q259R/Q280R/N28 2D/ N331S;
- 29) F31Y/S66P/Q97R/N113Y/K146Q/V181H/A215T/Q259R/Q280S/N282D/N33 1S;
- 30) S66P/Q97R/N113Y/N173T/V181A/A215T/Q259R;
- 31) F31Y/S66P/Q97R/N113Y/K146Q/N173H/V181A/A215T/Q259R/Q280S/N33 1S;
- 32) F31Y/S66P/Q97R/N113Y/V181H/A215T/Q259R;
- 33) S66P/Q97R/N113Y/N173H/V181A/A215T/Q259R/N331S;
- 34) F31Y/S66P/Q97R/N113Y/V181H/A215T/Q259R/Q280L;
- 35) F31Y/S66P/Q97R/N113Y/K146Q/V181H/A215T/Q259R/Q280L;
- 36) F31Y/S66P/Q97R/K146Q/V181H/A215T/Q259R/Q280R/N282D;
- 37) S66P/N113Y/V181H/A215T/Q259R/N282D;
- 38) F31Y/S66P/Q97R/V181H/A215T/Q259R/N282D;
- 39) S66P/N113Y/N173H/V181H/A215T/Q259R/Q280S/N331S;
- 40) F31Y/S66P/Q97R/N113Y/K146Q/N173H/V181H/A215T/Q259R/Q280S/N33 1S;
- 41) S66P/V181H/A215T/Q259R/N282D;
- 42) F31Y/S66P/Q97R/N113Y/K146Q/V181H/A215T/Q259R/Q280L/N331S;
- 43) S66P/Q97R/N113Y/N173H/V181H/A215T/Q259R/N282D;
- 44) S66P/Q97R/N113Y/V181H/A215T/Q259R/N282D;
- 45) S66P/V181H/A215T/Q259R;
- 46) S66P/Q97R/N113Y/V181H/A215T/Q259R/Q280R/N282D;
- 47) F31Y/S66P/N173T/V181H/A215T/Q259R/N282D;
- 48) F31Y/S66P/N113Y/V181H/A215T/Q259R/Q280R/N344D;
- 49) F31Y/S66P/Q97R/N113Y/N173H/V181H/A215T/Q259R/Q280R/N282D;
- 50) F31Y/S66P/Q97R/N113Y/N173H/V181H/A215T/Q259R/Q280S/N282D/N33 1S;
- 51) S66P/N113Y/N173H/V181H/A215T/Q259R/Q280S;
- 52) S66P/Q97R/N113Y/N173H/V181A/A215T/Q259R;
- 53) S66P/Q97R/N113Y/N173H/V181A/A215T/Q259R/Q280S;
- 54) S66P/N113Y/N173H/V181H/A215T/Q259R/Q280S/N282D;
- 55) F31Y/S66P/Q97R/N173H/V181H/A215T/Q259R/Q280R/N282D;
- 56) S66P/N113Y/N173H/V181H/A215T/Q259R;
- 57) S66P/Q97R/N113Y/N173H/V181A/A215T/Q259R/N331S;
- 58) F31Y/S66P/Q97R/N173H/V181H/A215T/Q259R/Q280S/N282D/N331S;
- 59) S66P/N113Y/N173H/V181H/A215T/Q259R/Q280S/N331S;
- 60) S66P/Q97R/N113Y/N173H/V181A/A215T/Q259R/Q280S/N331S;
- 61) F31Y/S66P/Q97R/Q149K/N173H/V181H/A215T/Q259R/Q280S/N331S;
- and
- 62) S66P/A215T/Q259R,
- and wherein the mannanase variant has a sequence identity of at least 90% to SEQ ID NO:
- 1.