DNA binding protein-polymerase chimeras
AssigneeAgilent Technologies, Inc.
InventorMichael Borns
Life of this patent
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Abstract
The invention relates to compositions and methods directed to chimeric DNA polymerases, which comprise a mutated DNA binding polypeptide domain and a mutated or wild-type DNA polymerase polypeptide domain.
Claims19
A chimeric DNA polymerase comprising a DNA binding domain and a polymerase domain, wherein said DNA binding domain has the wild-type sequence of Sso7d (SEQ ID NO:2) or of a wild-type Sso7d-like protein selected from the group consisting of Ssh7b (SEQ ID NO:8), RiboP3 (SEQ ID NO:9), Stole (SEQ ID NO:10), Sac7d (SEQ ID NO:12), Sac7e (SEQ ID NO:14), and Sac7a (SEQ ID NO:16), with the exception of seven, eight, or nine substitution mutations at positions corresponding to seven, eight, or all nine of the following amino acid positions:
- 13, 16, 40, 41, 45, 55, 56, 61, and 63 of SEQ ID NO:2.
A chimeric DNA polymerase comprising a DNA binding domain and a polymerase domain, wherein said DNA binding domain has the sequence of Sso7d (SEQ ID NO:2) or of a wild-type Sso7d-like protein selected from the group consisting of Ssh7b (SEQ ID NO:8), RiboP3 (SEQ ID NO:9), Stole (SEQ ID NO:10), Sac7d (SEQ ID NO:12), Sac7e (SEQ ID NO:14), and Sac7a (SEQ ID NO:16), with the exception of seven substitution mutations at the positions corresponding to amino acid positions 13, 16, 40, 45, 55, 56, and 63 of SEQ ID NO:2.
A chimeric DNA polymerase comprising a DNA binding domain and a polymerase domain, wherein said DNA binding domain comprises the amino acid sequence of SEQ ID NO:4.
A chimeric DNA polymerase comprising a DNA binding domain and a polymerase domain, wherein said DNA binding domain has the sequence of Sso7d (SEQ ID NO:2) or of a wild-type Sso7d-like protein selected from the group consisting of Ssh7b (SEQ ID NO:8), RiboP3 (SEQ ID NO:9), Stole (SEQ ID NO:10), Sac7d (SEQ ID NO:12), Sac7e (SEQ ID NO:14), and Sac7a (SEQ ID NO:16), with the exception of nine substitution mutations at the positions corresponding to amino acid positions 13, 16, 40, 41, 45, 55, 56, 61 and 63 of SEQ ID NO:2.
A chimeric DNA polymerase comprising the amino acid sequence of SEQ ID NO:20.
Dependencies are inferred from the claim text.