Method for increasing protein thermal stability
AssigneeJiangnan University
InventorsJian Chen, Guocheng Du, Xinyao Lu, Song Liu, Juan Zhang
Life of this patent
Estimating the term…
Abstract
The invention provides a simple and effective method for increasing thermal stability of a wide range of proteins, comprising fusing a self-assembling amphipathic peptide to the C- or N-terminal of target proteins. The fusion protein can have a half life up to 26 times longer than that of the wild type protein.
Claims2
A method for increasing thermal stability of a target protein, comprising fusing a self-assembling amphipathic peptide (SAP) having an amino acid sequence set forth in SEQ ID NO:5 to said target protein by recombinant techniques to make a Target Protein-SAP fusion protein, wherein said Target protein-SAP fusion protein has increased thermal stability.
Dependencies are inferred from the claim text.