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US 9,040,265 B2Granted
Oxidoreductases for the stereoselective reduction of keto compounds
AssigneeIep GmbH
InventorsAnke Tschentscher, Antje Gupta, Maria Bobkova
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AssigneeIep GmbH
InventorsAnke Tschentscher, Antje Gupta, Maria Bobkova
Estimating the term…
The invention relates to a process for the enantioselective enzymatic reduction of a keto compound to the corresponding chiral hydroxy compound, wherein the keto compound is reduced with an oxidoreductase in the presence of a cofactor, and is characterized in that an oxidoreductase is used which has an amino acid sequence in which (a) at least 70% of the amino acids are identical to the amino acids of one of the amino acid sequences SEQ ID No 1, SEQ ID No 6 and SEQ ID No 8, or (b) at least 55% of the amino acids are identical to the amino acids of the amino acid sequence SEQ ID No 2, or (c) at least 65% of the amino acids are identical to the amino acids of the amino acid sequence SEQ ID No 3, or (d) at least 75% of the amino acids are identical to the amino acids of the amino acid sequence SEQ ID No 4, or (e) at least 65% of the amino acids are identical to the amino acids of the amino acid sequence SEQ ID No 5, or (0 at least 50% of the amino acids are identical to the amino acids of the amino acid sequence SEQ ID No 7, or (g) at least 72% of the amino acids are identical to the amino acids of the amino acid sequence SEQ ID No 129.
A recombinant host cell which is a bacterial, insect, plant or isolated mammalian cell and has been transformed or transfected with an expression vector comprising:
An expression vector comprising:
A cloning vector comprising:
A recombinant host cell which is a bacterial, insect, plant or isolated mammalian cell and has been transformed or transfected with an expression vector comprising a nucleic acid sequence encoding an oxidoreductase having an amino acid sequence of at least 95% identical to SEQ ID No:6 or enzymatically active fragment thereof.
An expression vector comprising a nucleic acid sequence encoding an oxidoreductase having an amino acid sequence at least 95% identical to SEQ ID No:6 or enzymatically active fragment thereof.
A cloning vector comprising a nucleic acid sequence encoding an oxidoreductase having an amino acid sequence at least 95% identical to SEQ ID No:6 or enzymatically active fragment thereof.
A process for the enantioselective enzymatic reduction of a keto compound to the corresponding chiral hydroxy compound comprising contacting the keto compound with an oxidoreductase having an amino acid sequence at least 95% identical to SEQ ID NO:6 or enzymatically active fragment thereof and NADP or NADPH, wherein the keto compound is reduced to the corresponding alcohol.
Dependencies are inferred from the claim text.