Use of diazolidinyl urea for anti-clumping of biological samples
AssigneeBiocept, Inc.
InventorsPavel Tsinberg, Stephen D. Mikolajczyk
Life of this patent
Estimating the term…
Abstract
The present invention provides methods for preventing clumping of cells in microfluidic devices by addition of diazolidinyl urea (DU). DU can be added to samples at the time of collection or can be added to samples post-collection. DU can also be pre-added to sample collection devices.
Claims11
A method of inhibiting cellular aggregation in a biologically active sample comprising mixing an effective amount of diazolidinyl urea with a sample of viable cells, wherein the effective amount of diazolidinyl urea is in a concentration of about 0.01% to about 3% upon mixing with the sample of viable cells.
A method of inhibiting cellular aggregation in a biological sample comprising mixing an effective amount of diazolidinyl urea with the sample, wherein cellular aggregation is reduced in the presence of diazolidinyl urea compared to the absence of diazolidinyl urea in the sample, wherein the effective amount of diazolidinyl urea is about 0.01% to about 3% upon mixing with the biological sample, and wherein upon mixing with the effective amount of diazolidinyl urea the biological sample is suitable for use in a microchannel for cell capturing.
Dependencies are inferred from the claim text.