Method for enhancing recombinant protein production by cell-free protein expression system
AssigneeSutro Biopharma, Inc.
InventorsSushmita Mimi Roy, Sunil Bajad, Evan Green, Henry Heinsohn
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Abstract
The present invention provides a method for monitoring of profile changes of components in a dynamic system such as a cell-free in vitro protein synthesis system by using liquid chromatography (LC) combined with mass spectrometry (MS). In an additional aspect, this invention provides a method for enhancing the yield and/or reproducibility in a cell-free protein synthesis system by modulating the level and/or activity of a protein component that has regulatory effects on the system.
Claims4
A method for enhancing recombinant protein production in a cell-free protein synthesis system comprising:
- contacting a cell lysate prepared from prokaryotic cells comprising a recombinant genomic modification that abolishes CspE expression, and an exogenous nucleic acid template encoding a protein;
- and
- producing the protein encoded by the exogenous nucleic acid template in the cell-lysate.
Dependencies are inferred from the claim text.