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AssigneeCYANAGEN S.r.l.
InventorsLeopoldo Della Ciana, Federica Rodeghiero, Rossana Perciaccante
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Method for increasing and regulating the emission of light from a chemiluminescent reaction including luminol, a peroxidase enzyme, an oxidant and an electron mediator (primary enhancer) through the use of an acylation catalyst (secondary enhancer) belonging to the class of N-azoles, i.e., a class of five-membered nitrogen heteroaromatic ring compounds containing at least one other atom of nitrogen. N-azoles, which are especially useful as secondary enhancers are imidazole, 1-methylimidazole, 1,2,3-triazole and 1,2,4-triazole. The invention also describes the use in diagnostic assays of chemiluminescent substrates containing said N-azoles, as secondary enhancers.
A method for increasing the light emission produced by the chemiluminescent reaction of luminol, a peroxidase enzyme, an oxidant and a primary enhancer, wherein said primary enhancer is sodium 3-(phenothiazin-10-yl)propane-1-sulfonate, and wherein said chemiluminescent reaction occurs in the presence of a secondary enhancer wherein said secondary enhancer is selected from the group consisting of imidazole, 1-methylimidazole, 1,2,3-triazole, and 1,2,4-triazole.
A kit for performing an assay for determining an analyte in a sample, wherein the kit comprises luminol, an oxidant, a primary enhancer in a form of an electron mediator, wherein said primary enhancer is sodium 3-(phenothiazin-10-yl)propane-1-sulfonate, and a secondary enhancer is selected from the group consisting of imidazole, 1-methylimidazole, 1,2,3-triazole, and 1,2,4-triazole.
Dependencies are inferred from the claim text.