Biomolecule fixing board and method of manufacturing the same

A biomolecule modifying substrate comprising:

• a substrate;
• a first surface on the substrate, the first surface comprising a metal oxide;
• a second surface on the substrate, the second surface comprising a noble metal;
• a first linker molecule comprising a hydrocarbon chain and a functional group at one end or in a side chain of the hydrocarbon chain, the functional group capable of selectively binding to the first surface, the first linker molecule bound to the first surface via the functional group;
• a second linker molecule comprising biotin and a reactive group, the reactive group capable of binding to the hydrocarbon chain of the first linker molecule, the second linker molecule bound to the first linker molecule via a bond between the reactive group and the hydrocarbon chain;
• a biomolecule comprising an introduced biotin;
• and
• an avidin,
• wherein the biotin contained in the second linker molecule is bound to the biotin introduced into the biomolecule via the avidin, and
• wherein the functional group of the first linker molecule capable of selectively binding to the first surface is a phosphate group.