Kits for detecting and monitoring endocrine disrupting chemicals (EDCs)

A method for detecting or quantifying endocrine disruptor chemical (EDC) ligands in an environmental sample, the method comprising:

• contacting with the environmental sample:
• a first mammalian cell expressing a first traceable fusion protein;
• a second mammalian cell expressing a second traceable fusion protein;
• a third mammalian cell expressing a third traceable fusion protein;
• a fourth mammalian cell expressing a fourth traceable fusion protein;
• and
• a fifth mammalian cell expressing a fifth traceable fusion protein,
• wherein the first, second, third, fourth, and fifth traceable fusion proteins independently comprise either:
• (1) a superfamily receptor protein, and a marker protein domain;
• or
• (2) the cytoplasmic/nuclear translocation domain of glucocorticoid receptor, the ligand binding domain of a superfamily receptor protein, and a marker protein domain;
• and
• detecting cytoplasmic to nuclear translocation of the first, second, third, fourth, and/or fifth fusion proteins in response to one or more ligands of the ligand binding domain in the environmental sample,
• wherein the first, second, third, fourth, and fifth traceable fusion proteins each bind a different ligand or class of ligands.