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US 9,040,242 B2Granted
Method to amplify nucleic acids to generate fluorescence labeled fragments of conserved and arbitrary products
AssigneeE. I. DuPont de Nemours and Company
InventorsAnjana Agarwal, Mark A. Jensen
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Abstract
Disclosed herein are methods for the identification of the species, serotype, and strain of a microorganism. Also disclosed are primers for use in detecting such microorganisms and kits comprising such primers.
Claims8
A method for the identification of the species, serotype, and strain of a microorganism comprising:
- (a) amplifying DNA comprising variable sequences interspersed between highly conserved rDNA sequences by PCR and amplifying additional genomic sequences by random amplified polymorphic DNA (RAPD) PCR using a first primer of 13-14 bases in length and a second primer of 11-13 bases in length, said first primer comprising:
- (i) 11-12 contiguous bases from a highly conserved 16S rDNA region;
- (ii) 2 bases at the 5′ end of said first primer that are not complementary to said highly conserved 16S rDNA region;
- and
- (iii) a fluorescent label;
- and
- (b) separating the amplified DNA produced in step (a).
4 more elements
Dependencies are inferred from the claim text.