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US 9,040,239 B2Granted
Composition and methods of oxygenation of nucleic acids containing 5-methylpyrimidine
AssigneeNew England Biolabs, Inc.
InventorsYu Zheng, Lana Saleh, June Pais, Nan Dai, Richard J. Roberts, Ivan R. Correa, Jr., Megumu Mabuchi, Romualdas Vaisvila
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Abstract
5-methylpyrimidine oxygenases and their use in the modification of nucleic acids are described.
Claims27
A composition comprising:
- (i) a buffer comprising Fe(II) and alpha-ketoglutarate;
- and
- (ii) a polypeptide that has 5-methylpyrimidine oxygenase activity and has at least 90% identity to amino acids 154-304 of SEQ ID NO:
- 2.
1 more element
Dependencies are inferred from the claim text.