Recombinant bacterium capable of eliciting a protective immune response against C. perfringens

A recombinant attenuated Salmonella enterica bacterium, wherein the bacterium is capable of expression of one nucleic acid encoding at least three protein antigens of Clostridium perfringens, or wherein the bacterium is capable of expression of more than two nucleic acids, each encoding a Clostridium perfringens protein antigen, wherein the C. perfringens protein antigen(s) are selected from PlcC (C-terminal domain of C. perfringens alpha toxin), NetB, GDP (glyceraldehyde 3-phosphate dehydrogenase), PFOR (pyruvate:ferredoxin oxidoreductase), FBA (fructose 1,6-biphosphate aldolase), HP (a C. perfringens hypothetical protein), and a fragment thereof that is capable of eliciting an immune response, wherein the bacterium upon administration to a mammal or a bird, elicits an immune response against C. perfringens in the mammal or the bird.

A recombinant attenuated Salmonella enterica bacterium, wherein the bacterium is capable of expression of one or more than one nucleic acid, which encodes at least three protein antigens of Clostridium perfringens, wherein the protein antigens are PlcC (C-terminal domain of C. perfringens alpha toxin), NetB, and FBA (fructose 1,6-biphosphate aldolase) and wherein the bacterium upon administration to a mammal or a bird, elicits an immune response against C. perfringens in the mammal or the bird.