Marker genes for regulatory T cells from human blood
AssigneesUniversitaet Zu Koeln, Becton, Dickinson and Company
InventorsJoachim Ludwig Schultze, Marc Daniel Beyer, Noel Warner, Ravi Hingorani
Life of this patent
Estimating the term…
Abstract
The present invention provides novel marker genes for the specific identification and characterization of human suppressive and/or regulatory T cells including natural, adaptive, and expanded CD4+CD25+FOXP3+ T cells in healthy individuals as well as tumor patients or patients with autoimmune diseases.
Claims2
A method for isolating, identifying or characterizing suppressive and/or regulatory human T cells obtained from human blood, comprising:
- (a) contacting the human blood with one or more antibodies or antibody fragments that specifically bind to CD4, CD25 and/or CD127 on the T cells in order to isolate cells obtained from human blood;
- (b) contacting the cells obtained from human blood with one or more antibodies or antibody fragments that specifically bind to one or more marker proteins comprising a sequence consisting of SEQ ID NO:
- 8;
- and
- (c) isolating, identifying or characterizing antibody-bound or antibody-fragment-bound cells, wherein the antibody-bound or antibody-fragment-bound cells are suppressive and/or regulatory human T cells from the human blood.
A method for isolating, identifying or characterizing suppressive and/or regulatory human T cells obtained from human blood, comprising:
- (a) contacting the cells obtained from human blood with one or more antibodies or antibody fragments that specifically bind to one or more marker proteins comprising a sequence consisting of SEQ ID NO:
- 8;
- (b) isolating, identifying or characterizing antibody-bound or antibody-fragment-bound cells, wherein the antibody-bound or antibody-fragment-bound cells are suppressive and/or regulatory human T cells from the human blood;
- and
- (c) assaying for FOXP3 expression.
Dependencies are inferred from the claim text.