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US 9,040,038 B2Granted
Modalities for the treatment of degenerative diseases of the retina
AssigneeOcata Therapeutics, Inc.
InventorsIrina V. Klimanskaya, Robert P. Lanza
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Abstract
This invention relates to methods for improved cell-based therapies for retinal degeneration and for differentiating human embryonic stem cells and human embryo-derived into retinal pigment epithelium (RPE) cells and other retinal progenitor cells.
Claims4
A method of producing a composition comprising isolated human RPE cells suitable for human therapy, comprising:
- (a) culturing human pluripotent cells to form clusters of cells;
- (b) culturing the clusters of cells of step (a) under adherent conditions that do not maintain the undifferentiated state of the pluripotent cells for a sufficient time for the appearance of pigmented cells comprising brown pigment dispersed in their cytoplasm;
- and
- (c) isolating and culturing the pigmented cells to form a monolayer comprising cells having a cobblestone, polygonal, epithelial-like appearance and brown pigment dispersed in their cytoplasm, and expressing RPE65 and bestrophin, thereby obtaining human RPE cells.
1 more element
Dependencies are inferred from the claim text.