Production of tissue engineered digits and limbs
AssigneeWake Forest University Health Sciences
InventorsAnthony Atala, James J. Yoo, Grace Lim, Sang Jin Lee
Life of this patent
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Abstract
The invention pertains to methods of producing artificial composite tissue constructs that permit coordinated motion. Biocompatable structural matrices having sufficient rigidity to provide structural support for cartilage-forming cells and bone-forming cells are used. Biocompatable flexible matrices seeded with muscle cells are joined to the structural matrices to produce artificial composite tissue constructs that are capable of coordinated motion.
Claims11
A biocompatible composite scaffolding system capable of providing structural support for engineered bone tissue comprising:
- a first biodegradable matrix comprising a synthetic polymer and a naturally derived collagen matrix, wherein the first matrix has a porosity greater than about 50%;
- and comprises about 25% to about 75% synthetic polymer;
- a population of cells selected from the group consisting of cartilage-forming cells, bone-forming cells and combinations thereof seeded onto the first matrix;
- a second matrix comprising a flexible, collagen-containing matrix;
- and
- a population of muscle progenitor cells seeded onto the second matrix;
- wherein the seeded second matrix is preconditioned to induce muscle fiber orientation;
- wherein the first matrix and the second matrix are joined thereby forming a biocompatible composite scaffolding system.
Dependencies are inferred from the claim text.