Polymer coatings
AssigneeIllumina, Inc.
InventorsWayne N. George, Andrew A. Brown, Daniel Bratton, Hongji Ren, Ryan C. Smith
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Abstract
The present disclosure relates to polymer coatings covalently attached to the surface of a substrate and the preparation of the polymer coatings, such as poly(N-(5-azidoacetamidylpentyl)acrylamide-co-acrylamide) (PAZAM), in the formation and manipulation of substrates, such as molecular arrays and flow cells. The present disclosure also relates to methods of preparing a substrate surface by using beads coated with a covalently attached polymer, such as PAZAM, and the method of determining a nucleotide sequence of a polynucleotide attached to a substrate surface described herein.
Claims45
A substrate having a surface comprising a polymer coating covalently attached thereto, wherein the polymer coating comprises a recurring unit of Formula (Ia) and a recurring unit of Formula (II):
- wherein:
- R1 is selected from hydrogen, halo, alkyl, alkoxy, alkenyl, alkynyl, cycloalkyl, aryl, heteroaryl, heterocyclyl, or optionally substituted variants thereof;
- RA is azido;
- each R4, R4′, R5 and R5′ is independently selected from H, R6, OR6, —C(O)OR6, —C(O)R6, —OC(O)R6, —C(O)NR7R8, or —NR7R8;
- R6 is independently selected from H, OH, alkyl, cycloalkyl, hydroxyalkyl, aryl, heteroaryl, heterocyclyl, or optionally substituted variants thereof;
- each R7 and R8 is independently selected from H or alkyl, or R7 and R8 are joined together with the atom or atoms to which they are attached to form a heterocycle;
- each of the —(CH2)o can be optionally substituted;
- and
- o is an integer between 1-50.
A method of preparing a polymer coating immobilized to a surface of a substrate, said method comprising:
- contacting a polymer with a surface of a substrate, wherein the surface comprises a plurality of functional groups, thereby forming a layer of polymer coating on the surface, and wherein the polymer coating is covalently bonded to the functional groups on the surface,
- wherein the polymer coating comprises a recurring unit of Formula (Ia) and a recurring unit of Formula (II):
- wherein:
- R1 is selected from hydrogen, halo, alkyl, alkoxy, alkenyl, alkynyl, cycloalkyl, aryl, heteroaryl, heterocyclyl, or optionally substituted variants thereof;
- RA is azido;
- each R4, R4′, R5 and R5′ is independently selected from H, R6, OR6, —C(O)OR6, —C(O)R6, —OC(O)R6, —C(O) NR7R8, or —NR7R8;
- R6 is independently selected from H, OH, alkyl, cycloalkyl, hydroxyalkyl, aryl, heteroaryl, heterocyclyl, or optionally substituted variants thereof;
- each R7 and R8 is independently selected from H or alkyl, or R7 and R8 are joined together with the atom or atoms to which they are attached to form a heterocycle;
- each of the —(CH2)—o can be optionally substituted;
- and
- o is an integer between 1-50.
A method of preparing an array of beads comprising:
- forming a plurality of functional groups on the surface of one or more beads;
- and
- contacting a polymer coating with the beads to form a polymer coating layer on the surface of the beads, wherein the polymer coating is covalently bonded to the functional groups on the surface of the beads, wherein the polymer coating comprises a recurring unit of Formula (Ia) and a recurring unit of Formula (II):
- wherein:
- R1 is selected from hydrogen, halo, alkyl, alkoxy, alkenyl, alkynyl, cycloalkyl, aryl, heteroaryl, heterocyclyl, or optionally substituted variants thereof;
- RA is azido;
- each R4, R4′, R5 and R5′ is independently selected from H, R6, OR6, —C(O)OR6, —C(O)R6, —OC(O)R6, —C(O)NR7R8, or —NR7R8;
- R6 is independently selected from H, OH, alkyl, cycloalkyl, hydroxyalkyl, aryl, heteroaryl, heterocyclyl, or optionally substituted variants thereof;
- each R7 and R8 is independently selected from H or alkyl, or R7 and R8 are joined together with the atom or atoms to which they are attached to form a heterocycle;
- each of the —(CH2)—o can be optionally substituted;
- and
- o is an integer between 1-50.
Dependencies are inferred from the claim text.