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US 9,006,415 B2Granted
Targeted delivery of nucleic acids
AssigneeMassachusetts Institute of Technology
InventorsYin Ren, Sangeeta N. Bhatia
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Abstract
Aspects of the invention provide compositions and methods for delivering nucleic acids to target cells.
Claims31
A composition comprising:
- a polypeptide comprising a first homing domain and a second cell-penetrating domain, wherein the second cell-penetrating domain is N-terminal to the first homing domain and the N-terminus of the polypeptide is modified with a lipophilic group, and
- a nucleic acid, wherein the nucleic acid is non-covalently associated with the second cell-penetrating domain, and
- wherein the second cell-penetrating domain has two or more basic amino acids, and wherein the number of basic amino acids within the second cell penetrating domain is below a threshold level of amino acids above which the polypeptide targets cells non-specifically.
A polypeptide comprising:
- a first homing domain, and
- a second domain comprising a nucleic acid binding motif wherein the second domain is N-terminal to the first homing domain, and wherein the second domain has less than 25% basic amino acids, has protein transduction function, and is non-covalently associated with a nucleic acid and,
- wherein a saturated C12-C18 fatty acid is attached to the N-terminus of the second domain.
Dependencies are inferred from the claim text.