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US 9,006,394 B2Granted
Peptide-presenting surfaces for long-term culture of pluripotent cells
AssigneeWisconsin Alumni Research Foundation
InventorsLaura Lee Kiessling, Joseph R. Klim, Lingyin Li
Life of this patent
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Abstract
The present invention relates to methods of growing and maintaining pluripotent cells on an insoluble substrate that presents a peptide that binds to glycosaminoglycans, such as heparin. Specifically, methods of growing and maintaining pluripotent cells on substrates having a chemically defined surface presenting at least one peptide having basic amino acid residues separated by one or two hydrophobic amino acid residues.
Claims5
A composition comprising:
- an insoluble substrate having a surface that presents a GAG-binding peptide selected from the group consisting of SEQ ID NO:1 and SEQ ID NO:2;
- a culture medium that supports growth of pluripotent stem cells;
- and
- pluripotent stem cells adhering to the surface, wherein the cells display pluripotent cell-specific markers and an ability to differentiate into all three germ layers after more than three months of adhering to the surface.
1 more element
Dependencies are inferred from the claim text.