Method of isolating analytes from a sample
AssigneeGeorge Mason Research Foundation, Inc.
InventorsLance Liotta, Emanuel Petricoin, David Geho
Life of this patent
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Abstract
The current invention is a capture-particle comprising: a) a molecular sieve portion; and b) an analyte binding portion; wherein the molecular sieve portion, analyte binding portion or both further comprise a cross-linked region having modified porosity. Capture particles wherein the molecular sieve portion, analyte binding portion or both comprise pore dimensions sufficient to exclude molecules larger than about 60 kDa. These particles are useful in purification and diagnostic methods. Kits comprising the capture particles are also described.
Claims16
A capture-particle consisting of:
- a) an outer molecular sieve portion;
- and
- b) an inner analyte binding portion with reactive organic affinity molecules configured for binding and sequestering analytes such that there are no dyes used for signaling detection;
- wherein the molecular sieve portion, analyte binding portion or both further comprise a cross-linked region having porosity physically configured to permit passage of analytes of a defined molecular mass or weight such that there is no chemical cleaving.
A kit for the diagnosis, prognosis or monitoring of a disease state consisting of:
- a container for collecting a fluid comprising analytes indicative of said disease state;
- and
- an amount of capture-particles for uptake and removal of said analytes, said capture-particles consisting of:
- a) an outer molecular sieve portion;
- and
- b) an inner analyte binding portion with reactive organic affinity molecules configured for binding and sequestering analytes such that there are no dyes used for signaling detection;
- wherein the molecular sieve portion, analyte binding portion or both further comprise a cross-linked region having porosity physically configured to permit passage of analytes of a defined molecular mass or weight such that there is no chemical cleaving.
Dependencies are inferred from the claim text.