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US 9,005,975 B2Granted
Method for selecting clone of induced pluripotent stem cells
AssigneeKyoto University
InventorsHideyuki Okano, Yohei Okada, Shinya Yamanaka, Kyoko Miura
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Abstract
To efficiently identify and select a clone from clones of induced pluripotent stem cells (iPS cell) having low tumor formation rate in vivo when allowed to differentiate and transplanted in a living body, iPS cells of the clones are induced to differentiate, undifferentiated cells among the cells after the induction of differentiation are detected, and a clone having the content of the undifferentiated cell below a control is selected.
Claims4
A method for selecting induced pluripotent stem cells (iPS cells) comprising:
- (1) providing iPS cell clones,
- (2) differentiating the iPS cell clones into neurospheres,
- (3) measuring the percentage of cells that express Nanog gene in the neurospheres, and
- (4) selecting iPS cell clones corresponding to neurospheres measured in step (3) having decreased percentage of cells expressing Nanog as compared to neurospheres obtained from embryonic stem (ES) or iPS cell clones known not to cause tumor formation when differentiated and transplanted into a living body.
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