Method of culturing cells under regulation in the extension direction

A method of culturing cardiac myocytes, comprising

• providing cardiac myocytes and culture medium to a cell culture plate and culturing the cardiac myocytes in the cell culture plate to form an interconnected cardiac muscle sheet which pulsated synchronously,
• wherein the cell culture plate comprises a plurality of side walls and a plurality of space structures for arranging the cardiac myocytes which are formed by the plurality of side walls, wherein the plurality of side walls comprise openings configured to provide a communicative structure that allows communication of the plurality of space structures to thereby induce the formation of desmosomes, which serve as scaffolds in cell culture, on the side walls, whereby the cardiac myocytes cultured in each space structure extend toward the opposing side walls and the cardiac myocytes cultured in the respective space structures are connected through the openings that directionally regulates extension of the cultured cardiac myocytes,
• wherein the cardiac myocytes are distributed over the area of the plurality of side walls and the plurality of space structures.